Dna binding studies of metal complexes pdf

The results suggest that both complexes bind to dna through intercalation and enantioselectively interact with ctdna. Both schiff bases and other metal complexes were found to be inactive. Detailed dna binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Synthesis, structural characterization, antimicrobial and. Dna interactions and biocidal activity of metal complexes indian. Synthesis, characterization, antimicrobial, dna binding. Design, syntheses and functional studies of dna binding metal complexes final seminar april 10, 1998 antitumor activity of many anticancer drugs is based on their interaction with dna five basic interaction types are observed. Morphological studies were carried out by scanning electron microscopic method.

Design, structural characterizations, dna binding, dft calculations, molecular docking and. Dna binding studies the binding interactions of metal complexes with dna were studied by electrochemical method using different concentrations of ctdna. Synthesis, characterization and dna binding study of ruthentumii thiosemicarbazone complexes k. They also can be served as complementary studies for the corresponding complexes of ruthenium. The dna cleavage activity of the schiff base metal complexes was monitored by agarose gel electrophoresis on ctdna. Stability, energetics and dna binding studies of zinc. In recent years, binding studies of transition metal complexes have become. Synthesis, structural, dna binding and cleavage studies of cu.

The characterization studies of all the metal complexes have been done with appropriate methods. Pdf synthesis and dna binding studies of ni ii, co ii. The complexes are characterized by ftir, uvvisible and mass spectral studies and assessed for their dna binding study using. Fluorescence scatchard plots demonstrated that the palladiumii complexes act as dualfunction metallointercalators and bind. Thus, the current study is focused on synthesis of zinc ii complexes using amino acid schiff bases as ligands. These studies are necessary for the further comprehension of binding of transition metal complexes to dna. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schiff base metal complexes.

Design, syntheses and functional studies of dna binding metal. Metallointercalators with different metal centers, oxidation states, coordination geometries, and overall sizes will exhibit varying depths of insertion. These studies revealed that the complexes showed an intercalative mode of binding to ct dna and also effectively cleaved the supercoiled. Competitive dna binding studies between metal complexes, cuphendionphenclcl 1, cuphendionebpyclcl 2, cudppt2h2opf6 3, nidppt2cl2 4, zndppt2cl2 5, and k3fecn6 6 where phendione. Spectral titration assessed the relative noncovalent binding of metal complexes to dna and the results are corroborated with each other techniques. The dna cleavage studies of metal complexes presented more protruding activity in the attendance of h 2 o 2 associated to that in the absence of h 2 o 2. These complexes are lowenergy mlct absorbers and bind to dna rapidly. The interaction of transition metal complexes containing multidentate ligands with dna has recently gained. Structural data of dna binding and molecular docking studies. Based on elemental and spectral data, the complexes were found to be of coc3h4n24clo42 1, fec3h4n23clo43 2 and mnc3h4n24ac2 3 c3h4n2 imidazole. Elemental analysis table 1 shows that the ligands and the metal salts are in the ratio of 2.

Stability, energetics and dna binding studies of zinc complexes. Synthesis, characterization, dna binding interaction. Binding studies of metalsalphen and metalbipyridine. Drug based oxovanadiumiv complexes, mic, intrinsic binding constants k b, gel electrophoresis and viscometric techniques, antimicrobials introduction the interaction of transition metal complexes contain. Synthesis, characterization and antimicrobial screening. Studies on dna binding to metal complexes of sal 2trien. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well. Dna binding studies were performed on h 2 l and its three complexes by means of electron absorption titration and ebdna competition experiments, and the results indicate they all bind dna in an intercalation mode, and their binding affinity follows the order of 1 2 3 h 2 l. Reactivity and structural studies of metal complexes bound.

These studies confirm that the complex 1 binds to ctdna through intercalation. Spectroscopic, microbial and dna binding affinities of. The experimental results show conformably that the mode of binding of the complex fesal2trienno3 to dna is nonclassical electrostatic action. Noncoordinative interactions with dna duplexes, quadruplex structures and other secondary structures are being exploited by mono and polynuclear metal complexes. Synthesis, characterisation and dna binding studies of metal. Dna binding and cleavage studies of macrocyclic metal. Pushpa latha the allen institute for ai proudly built by ai2 with the help of our collaborators using these sources. Truly, metal complexes bound to dna provide an interesting system to study structure, function and dynamics in a single package. Dna binding studies of the complexes were investigated by uvvis, fluorescence, thermal melting and viscosity measurements.

Electronic absorption spectroscopy serves as the most common means to study the interactions between metal complexes and dna barton et al. Competitive dnabinding studies between metal complexes, cuphendionphenclcl 1, cuphendionebpyclcl 2, cudppt2h2opf6 3, nidppt2cl2 4, zndppt2cl2 5, and k3fecn6 6 where phen. The cytotoxicity studies of 1 and 2 complexes were discussed in this paper. Proteins are gaining importance as nontraditional targets for metal complexes either by using the metal as a structural scaffold or by exploiting the reactivity of coordination. A series of novel complexes of transition metal co, fe and mn with imidaloze have been synthesized and characterized by elemental analysis, ftir and uvvis absorption spectroscopy. Dna binding ability of these complexes was studied on ctdna by using uvvis absorption, fluorescence and viscometry. All materials were purchased and used without further purification. Synthesis, characterization, and dna binding properties of a. The concentration of calfthymus ct dna was determined from the absorption intensity at 265 nm with a e value li et al. All the metal complexes were screened for antibacterial activities. Here, this problem is explored in the context of the design and reactivity of different metallointercalators bound to dna. Post graduate and research department of chemistry, sri ramakrishna.

In concordance with the cytotoxicity studies, the dna binding studies. Also, bioactive metal complexes are useful biochemical tools for the detection of dna both in vitro and inside the cell, as dna probes 14, 15. From the results, it can be concluded that both the complexes show intercalative binding mode with dna rather than groove binding. Synthesis, characterization, dna binding interaction studies. Among the complexes cadmium complex showed higher inhibition against the fungi candida albicans.

Studies on dna binding to metal complexes of sal2trien. Synthesis, structural, dna binding and cleavage studies of. Jun 19, 2003 a series of mixed metal complexes coupling a lightabsorbing ruii terpyridine unit to a reactive cisptiicl2 moiety through polyazine bridging ligands have been prepared and their light absorbing, redox, and dna binding properties assayed. Synthesis and dna binding studies of niii, coii, cuii and znii metal complexes of n1,n5bispyridine2 methylene thiocarbohydrazone schiffbase ligand. Thus through a series of experiments in this thesis, attempts have been made to correlate the structure and dynamics of metal complexes bound to dna. Synthesis, characterization, biological activity and dna. The complexes fesal2trienno3 and cusal2trien have been synthesized and their interaction with calf thymus dna has been investigated for the first time using uv spectra, fluorescence spectra, thermal denaturation, and viscosity measurements. Metallointercalators insert themselves between two intact base pairs without expelling or replacing the original nitrogenous bases. M of hydrogen peroxide in 50 mm trishcl buffer ph 7.

The interaction of metal complexes with biomolecules has been extensively studied in recent decades. Dna binding studies the binding interactions of metal complexes with dna were studied by electrochemical method using different concentrations of ct dna. Synthesis and dna binding studies of ni ii, co ii, cu ii and zn ii metal complexes of n 1, n 5bis pyridine2methylenethiocarbohydrazone schiffbase ligand. Dna binding studies the dna binding experiments were performed in trishcl nacl buffer 50 mm trishcl1 mm nacl buffer, ph 7. For example, square planar complexes penetrate deeper into the dna. Synthesis, characterization, and dna binding properties of. We have also investigated the interaction between the complexes and a sequence of duplex dna to gain some insight into the selectivity of the compounds for. Design, structural characterizations, dna binding, dft calculations, molecular docking and biological studies shadia a. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a duplex dna have been determined. Dna binding studies were carried out by viscosity method. Dna binding and cleavage studies electronic absorption titration. The % fluorescence decrease is directly related to the extent of dna binding. Read synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone, journal of fluorescence on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

The photochemical dna cleavage of the complexes is also demonstrated. Dna binding studies of rutheniumii dipyridotetrahydrophenazine complexes dna binding studies of rutheniumii dipyridotetrahydrophenazine complexes howard, warren a aldrichwright, janice r. Pdf metal complex interactions with dna researchgate. Studies on dna binding to metal complexes of sal 2trien zhou chengyong1,2, xi xiaoli 1, and yang pin1 1key laboratory of chemical biology and molecular engineering of the ministry of education, institute of molecular science, shanxi university, taiyuan, shanxi 030006, pr china. As zinc being an active core of binding site of some metalloenzymes, the synthesis of structurally similar zinc ii complexes is on the offing. Tuned structure and dna binding properties of metal. Dna binding and cleavage studies of two palladiumii. Aug 27, 2016 dna binding ability of these complexes was studied on ct dna by using uvvis absorption, fluorescence and viscometry. Competitive binding studies with ethidium bromide have shown that both complexes strongly bind to fsdna, and the apparent binding constant k app calculated from emission studies was 2. Synthesis, characterization, antimicrobial activity and. Synthesis, characterization, dna binding properties. Synthesis, characterisation and dna binding studies of.

The cyclic voltammograms of the complexes in the presence of ctdna show that the complexes can bind to ctdna by intercalative and electrostatic binding. The binding constants of complexes have been determined by the absorption titration spectrofluorimetric method. Dna binding studies were also carried out for the synthesized complexes. Synthesis, characterization and dnabinding studies of. Based on elemental and spectral data, the complexes were found to be of coc 3 h 4 n 2 4 clo 4 2 1, fec 3 h 4 n 2 3 clo 4 3 2 and mnc 3 h 4 n 2. Reactivity and structural studies of metal complexes bound to dna. Dnabinding and photocleavage studies of cobalt iii. The complexes have shown to interact strongly with quadruplex dna with binding constants of ca. Request pdf studies on dna binding to metal complexes of sal2trien the complexes fesal2trienno3 and cusal2trien have been synthesized and.

Jun 20, 2016 the focus of this work is introduction of gelred gr as a stable, sensitive and environmentally safe fluorescent dna dye instead of the highly toxic ethidium bromide eb. Studies on dna binding to metal complexes of sal 2 trien. Competitive dnabinding studies between metal complexes and. Dpph assay the free radical scavenging activity of the ligand l and its complexes were determined using 2,2diphenyl1picrylhydrazyl dpph method 4.

Synthesis, characterization and dna binding study of mixed. Dna binding studies of metal complexes mn ii, co ii, ni ii, cu ii to dna helix has been characterized through absorption spectral titrations, significant hypochromism with a red shift of 10 nm bathochromism of absorption band implicates intercalative mode of binding and is likely that the all complexes with aromatic chromophore. Synthesis, characterization and dna binding study of. Pdf synthesis and dna binding studies of niii, coii. In continuance of our ongoing research on dna binding and cleavage happenings of transition metal complexes, in this paper we obtainable the synthesis, characterization and dna cleavage.

Since the discovery of cisplatin, transitional metal complexes have been proven to be potent dna intercalating agents. Pdf synthesis and characterization of metal complexes. Synthesis, characterization and biological evaluation of. Synthesis, characterization, crystal structure and dnabinding. All the experiments involving interaction of complexes with ctdna were carried out in a buffer containing 5 mmol of trishydroxymethylaminomethane and 50 mmol of nacl adjusted to ph 7. Taking into account the analytical and spectral studies, an octahedral geometry is assigned to all these complexes. Synthesis, characterization, biological activity and dna binding studies of metal complexes with 4aminoantipyrine schiff base ligand b. Jayabalakrishnan2 1department of chemistry, kumaraguru college of technology, coimbatore, tn, india 2department of chemistry, sri ramakrishna mission vidyalaya college of arts and science, coimbatore,tn, india. The dna binding and cleavage study of the copper complex containing the ligand is reported. Structural data of dna binding and molecular docking.

The focus of this work is introduction of gelred gr as a stable, sensitive and environmentally safe fluorescent dna dye instead of the highly toxic ethidium bromide eb. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schiff base metal complexes by poomalai jayaseelan, selladurai prasad, subramanian vedanayaki and. Ii complexes to dna, competitive binding experiments were carried out. Competitive dnabinding studies between metal complexes. Pdf synthesis, characterization, dna binding properties. Selective binding of transition metal complexes to dna introductionab initio calculations and photocleavage studiesnmr spectroscopic studies of metal binding to dna. In vitro study on dna binding of rutheniumii complexes. Dna binding and photocleavage studies of cobaltiii.

Synthesis, spectral, dna binding and cleavage properties of. Pdf synthesis and dna binding studies of niii, coii, cu. Dna binding and cleavage studies of macrocyclic metal complexes containing heteroatomic ligands neha mathur and sonlata bargotya department of chemistry, govt. Design, syntheses and functional studies of dna binding. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy. Establishing correlations among structure, dynamics and reactivity is a fundamental problem in biological chemistry. Thus, the current study is focused on synthesis of zinc ii. Furthurdna binding studies were carriedout for the complexes.

First, the effects of intervening mismatches on dna structure, dynamics and dna charge transport reactivity is examined. Request pdf studies on dna binding to metal complexes of sal2trien the complexes fesal2trienno3 and cusal2trien have been synthesized and their interaction with calf thymus dna has. Hemolytic and dna binding studies of divalent transition. Further dna was isolated from yeast cells and checked for purity, dna binding ability of both the complexes was studied by uv absorption titration technique. Studies on dna binding to metal complexes of sal 2trien zhou chengyong1,2. The tests were performed under aerobic conditions with h 2 o 2 as an oxidant. The method is based on the decrease of fluorescence derived from the displacement of gelred from dna by metal complexes. Synthesis, characterization, antimicrobial and dna binding. The mobility of the bands decreased as the concentration of the complex was increased, indicating that there was increase in the interaction between the metal. Dna cleavage, binding and intercalation studies of drugbased oxovanadiumiv complexes m. The binding affinities of these complexes with calf thymus dna are also reported. Synthesis, characterization, analytical properties, and dna binding studies article in synthesis and reactivity in inorganic metalorganic and nanometal chemistry 4410. Synthesis, characterization, dna binding and cleavage studies.

They are found to be potent dna intercalating and cleaving agents. The relative binding efficacy of the complexes to dna was much higher than the binding of ligands, especially the complex of cu l1 l2 cl 2 had the highest binding ability to dna. From all the spectral data a distorted octahedral geometry has proposed for both the complexes. Synthesis, characterization, antimicrobial and dna.

Synthesis, characterization, antimicrobial, dna binding and. A series of mixedmetal complexes coupling a lightabsorbing ruii terpyridine unit to a reactive cisptiicl2 moiety through polyazine bridging ligands have been prepared and their light absorbing, redox, and dna binding properties assayed. Tga revealed that the synthesized compounds were thermally stable. In addition, dna binding properties of the ligand and its metal complexes have been investigated by electronic absorption spectroscopy, fluorescence spectra, ethidium bromide displacement experiments, iodide quenching experiments, salt effect and viscosity measurements.

Synthesis, characterization, crystal structure and dna. Synthesis and dna binding study of zinc ii coordination. Dna cleavage ability of the complexes was examined on pbr322 dna by using gel electrophoresis method. Dna cleavage, binding and intercalation studies of drugbased. Synthesis, structural characterization, and quadruplex dna. Hence the present study aims for the synthesis, characterization, antimicrobial and dna binding studies of manganese and zinc complexes containing mixed ligands 8. Results indicate the dna binding affinities of complexes follow the order 3 4 1 2 5 6. Hence the present study aims for the synthesis, characterization, antimicrobial and dna binding studies of manganese and zinc complexes containing mixed ligands 8hydroxyquinoline and 110 phenanthroline. The interactions between the metal complexes and dna were studied using electronic absorption method. All the dna binding studies reveal that they are good intercalators. The binding properties of the two complexes to calf thymusdna ctdna were investigated by different spectrophotometric methods and viscosity measurements together with equilibrium dialysis and circular dichroism spectroscopy. The photochemical dna cleavage of the two complexes is also demonstrated. These studies are necessary for further comprehension of binding of transition metal complexes to dna. Hence, we report synthesis and characterization of novel schiff base ligand l and its complexes with cuii.

Apr 09, 20 competitive binding studies with ethidium bromide have shown that both complexes strongly bind to fsdna, and the apparent binding constant k app calculated from emission studies was 2. Dna cleavage, binding and intercalation studies of drug. Synthesis, characterization, antimicrobial activity and dna. In vitro study on dna binding of rutheniumii complexes with. Dnabinding metallointercalators are positively charged, planar, polycyclic, aromatic compounds that unwind the dna double helix and insert themselves between dna base pairs. Binding of metallointercalators to dna is reversible and depends on the properties of the intercalating molecule. Dna binding studies of metal complexes mn ii, co ii, ni ii, cu ii to dna helix has been characterized through absorption spectral titrations, significant hypochromism with a red shift of 10 nm bathochromism of absorption band implicates intercalative mode of binding and is likely that the all complexes with aromatic chromophore stabilizes the dna duplex.